Your upright research microscope for advanced imaging. Various manual microscope components objective, optovar, camera adapter may be. The leica sp5 laser scanning confocal system is used for advanced multichannel fluorescence and livecell applications. Beneath the apotome model is a cutaway view of the light path showing the. Axiovert 200 notes on instrument safety carl zeiss b 40080 e 0301 07 notes on instrument safety the axiovert 200 axiovert 200 m microscopes were designed, produced and tested in compliance with. Zen lets you import images and data from 3rdparty microscope instruments so it stays at all times in the center of a homogenized processing, analysis and viewing workflow across. When finishing session, please disengage the apotome.
The grid is inserted into the light path of the microscope and uses the epiilluminator lens system to project a shadow of the grid lines into sharp focus, superimposed on the. Training manuals institute for molecular bioscience. For a description of the full functionality please refer to the manual or help file accompanying the. Zeiss axio imager z2 upright microscope manual duke. They are versatile in their use and deliver good optical quality. Anybody have any experience with the zeiss apotome structured illumination system. This system has five lasers generating nine lines for fluorescence excitation 405. This operating manual describes the use of the apotome. The aim of these instructions is to show you how to calibrate the apotome for a. With apotome for axio imager, the new generation of light microscopes has set a new standard in 3d imaging. Zeiss microscopy online campus interactive tutorials. These devices provide a powerful means to eliminate from images the. Apotome module must be placed in its slot which is on the right side. The axiovision software allows automatic collection of multichannel zstack images.
Zeiss fluorescence microscope with apotome attachment axio. Image acquisition robarts research institute apotome training manual july 2014 objective na immersion working distance coverglass thickness 10x plan neofluar 0. Training manual zeiss apotome wide field microscope. Zeiss opmi vario surgical microscope service manual 8. User manuals, zeiss microscope operating guides and service manuals. With structured illumination, you know that only the focal plane appears in your. Beneath the apotome model is a cutaway view of the light path showing the aperture grid and the rotating optical glass plate that directs a projection of the grid across the specimen. Artefact analysis and reduction utilizing a parameter optimization.
Zeiss fluorescence microscope with apotome attachment axio imager z1 stand this widefield microscope has filter cubes for dapi, cfp, gfp, yfp, texas red and cy5. This system has the capability to acquire multichannel, zstack and timelapse images. With apotome for axio imager, the new generation of light microscopes has set a new standard in 3d. Zeiss axio imager z2 upright microscope manual duke light. Confocal 2 zeiss 880 invert with fast airyscan training manual to follow confocal 3 zeiss 710 upright training manual. You are responsible for the condition of the microscope at the close of your session. The zeiss axioimager a1 brightfield microscope is the workhorse microscope for the collection of histochemically and immunohistochemically stained samples. Training manual zeiss apotome wide field microscope july 2014. No part of this document may be reproduced or dist ributed in any form or by any means without the prior written consent of carl zeiss microimaging gmbh. Optical sections by means of structured illumination. Exporting images from zeiss czi format to tif files using. Fluar the photon collectors the objectives of the fluar series are manufactured from special optical glasses. Zeiss stereo discovery v12 stereo microscope instruction manual english. Zeiss axiovert 25 microscope manual pdf service manual axiovert 40 number one in its class axiovert 25 c, axiovert 25 zeiss axiophot microscope user carl zeiss axiovert 25, axiovert 25 cfl b.
For notes and instructions for the control of the apotome. Worldwide supplier of microscopy and laboratory equipment. Carl zeiss microimaging gmbh accepts no liability for the performance or use of such products. Zeiss training and education expand your possibilities practical microscopy training has a long tradition at zeiss. Objectives from carl zeiss exceeding your expectations. Axiovert 200 notes on instrument safety carl zeiss b 40080 e 0301 07 notes on instrument safety the axiovert 200 axiovert 200 m microscopes were designed, produced and tested in compliance with din 610101 iec 10101, safety requirements for electrical measuring, control and laboratory. Clicking on the links below will either open or download a. I am writing a proposal for a new microscope system and the vendor mentioned. This zeiss technology uses structured illumination and computation to cut off scattered light from outoffocus planes, which generates images with enhanced contrast and higher resolution.
Nov 06, 2018 clicking on the links below will either open or download a. Zeiss axioimager a1 brightfield microscope ucsf drc. Download brochures and interactive product information for carl zeiss microscopes. Hitech instruments is a full service microscope and imaging company, serving the scientific community as a leading supplier of microscopes, digital imaging systems, image analysis software and automation equipment since 1976. Operating manual axiovert 200 axiovert 200 m inverted. Carl zeiss as upright microscopes axio imager 2 axio.
Can system operator panel sycop 3 with controller ems 3 option. Exporting images from zeiss czi format to tif files using zen 2. Please contact the zeiss service department or carl zeiss microscopy service to repair the instrument. Operating manual axio observer inverted microscope bioflux. No part of this document may be reproduced or dist. Axio imager z1 motorized version with highprecision z drive with 6 objective lens10x, 20x, 40x, 40x oil, 63x oil and 100x oil. Confocal scanning microscopy, a form of optical sectioning microscopy, has radically transformed optical imaging in biology.
Know more about our imaging solutions and important key applications for microscopy. Leica sp5 laser scanning confocal microscope ucsf drc. Microscope stand axio observer 3 materials and 5 materials, manual t 5 ah 250 v, 5x20 mm power supply unit vp2322 for axio observer t 4. Axio imager z1 motorized version with highprecision z drive with 6 objective. The tutorial initializes with an animation of the zeiss apotome in action featured in the upper lefthand side of the window.
Instructions, manuals, brochures and catalogues if you buy a used microscope, it probably wont be supplied with an instruction manual, and most manufacturers dont keep copies of manuals for. Zeiss apotome fluoro 5 quickstart guide for zen v2. Apotome training manual july 2014 1 2 3 work area image window tool bar. Instructions, manuals, brochures and catalogues if you buy a used microscope, it probably wont be supplied with an instruction manual, and most manufacturers dont keep copies of manuals for discontinued microscopes. I am writing a proposal for a new microscope system and the vendor mentioned this as a. Updated on april 29, 2011 apotome fluorescence microscope makemodel. The principle behind the apotome implementation of structured illumination is presented in the tutorial. Zeiss fluorescence microscope with apotome attachment. A grid created by evaporating metal on the surface of an opticalgrade glass window is inserted into the field diaphragm plane in the illumination pathway of a fluorescence microscope and projected onto the specimen. Page 22 installation stereo microscope zeiss installing the stereo microscope stemi 305 insert the connection cable fig. The first courses were held in jena as early as 1907, initiated by two notable zeiss scientists, henry siedentopf and august koehler. Assorted list of old manuals and catalogues including zeiss modelw, standard, universal, photomicroscope etc. Hitech instruments is a full service microscope and imaging company, serving the scientific community as a leading supplier of microscopes, digital imaging systems, image analysis software and. The condenser is set up for darkfield and dic optics.
Resolution in the apotome and the confocal laser scanning. With structured illumination, you know that only the focal plane appears in your image. The basic concept behind the zeiss apotome is the use of an evenly spaced grid in the aperture plane to serve as a mask through which the specimen is illuminated. The eyepiece cover, the plastic encapsulation must be in the right place and the apotome module must be placed in its slot which is on the right side of the microscope. The first courses were held in jena as early as 1907, initiated by two notable zeiss. V16 microscope body binocular ergo phototube z 030 mot with pl 16x16 eyepieces stand base 450 focus motor 3 with profile column objective nosepiece z, 2x cod mechanical stage s 150x100 mot. If you find it dirty, make sure microscopy staff know about it. Overview the zeiss apotome is an upright compound microscope with multiple automated functions. The courses have since been continuously advanced and updated. Zeiss efficient navigationzen, for short is the one user interface you will. Oct 12, 2014 assorted list of old manuals and catalogues including zeiss modelw, standard, universal, photomicroscope etc. Carl zeiss objectives database 22 overview aplan the a class aplan objectives from carl zeiss offer sound and reasonably priced entry into the world of microscopy. Zeiss standard wl research microscope brochure german.